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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which results in chromosome instability, and therefore may add to tumorigenesis. This protein was found to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a essential role in promoting cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually explained. Two further splice variants were documented but in-depth nucleotide sequence info is not nonetheless available.
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Blocking peptides are peptides that bind exclusively on the concentrate on antibody and block antibody binding. These peptide normally is made up of the epitope recognized through the antibody.
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This guide outlines additional attainable optimization of mobile and immunoassay parameters to be sure the very best final results are...
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This guide outlines additional possible optimization of cellular and immunoassay parameters to make certain the absolute best final results are...
Antibodies sure to the blocking peptide not bind into the epitope around the concentrate on protein. By comparing the staining from your blocked antibody versus the antibody by itself, one can see which staining is precise.
Target Info Cyclin E belongs to your really conserved cyclin household, whose users are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add to the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is degraded as cells development by means of S stage.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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